I bottle my kombucha in 16 oz bottles with 4 oz of juice for flavor and to provide the sugar for carbonation. I add this yeast to the batch at bottling time, about 1/4 or less of a packet per gallon of kombucha to bottle. I get very good carb in 4-5 days (I use a few plastic seltzer bottles to monitor the carb level of the batch). This yeast continues to work, very slowly, in the fridge. The last bottles of my batches are always very, very carbed. spritzy.